RNA formaldehyde gels: Difference between revisions
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Clean gel box and combs etc with detergent, then dionized water.
Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored in cold room) for 30 minutes.
Rinse box thoroughly with DEPC water.
Your should pour (and ideally run) the gel in the hood, since the gel contains formaldehyde. [see protocol from Maniatis (original edition)—attached.]
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<li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored in cold room) for 30 minutes. | <li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored in cold room) for 30 minutes. | ||
<li>Rinse box thoroughly with DEPC water. | <li>Rinse box thoroughly with DEPC water. | ||
<h3>For making gel and preparing samples:</h3> | |||
<li>Your should pour (and ideally run) the gel in the hood, since the gel contains formaldehyde. [see protocol from Maniatis (original edition)—attached.] | |||