Serum immunostaining: Difference between revisions
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For detailed information see Heat Inactivation of Serum. | For detailed information see "Heat Inactivation of Serum". |
Revision as of 15:54, 7 November 2012
Responsible person
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Protocol
The following protocol applies to Gibco Goat Serum from Invitrogen (Ordernr.: 16210).
- Defrost the serum (takes a long time- maybe overnight in the fridge is practical)
- Heat inactivate for 30 min at 56C
- Cool on ice and aliquot into 50ml Falcon tubes.
- Keep at -20 C
For detailed information see "Heat Inactivation of Serum".