Preparation of Freezing media for A1 cells: Difference between revisions
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== | == Material == | ||
* DMSO (one ampules from Sigma) | * DMSO (one ampules from Sigma) | ||
* High serum for A1 cells | * High serum for A1 cells | ||
| Line 8: | Line 8: | ||
== Procedure == | == Procedure == | ||
# clean the ampuls with ethanol | |||
# transfer the DMSO into the 15 ml FALCON tube | |||
# pipette in each 50 ml FALCON tube 18 ml High serum | |||
# put into each 2 ml DMSO and mix | |||
# aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube) | |||
# store the tubes into –20ºC | |||
Latest revision as of 14:28, 8 June 2015
Material
- DMSO (one ampules from Sigma)
- High serum for A1 cells
- 2x 50 ml FALCON tubes
- 1x 15 ml FALCON tube
- Pipettes
- 10x 5 ml FALCON tubes
Procedure
- clean the ampuls with ethanol
- transfer the DMSO into the 15 ml FALCON tube
- pipette in each 50 ml FALCON tube 18 ml High serum
- put into each 2 ml DMSO and mix
- aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube)
- store the tubes into –20ºC