Preparation of Low Serum for A1 cells: Difference between revisions
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(Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce...") |
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== | == Material == | ||
* 280 ml MEM | |||
* 100 ml sterile water | |||
* 1 tube Pen/ Strep (4ml) | |||
* 1 tube Glutamine (4ml) | |||
* 1 tube Insulin (4 ml) | |||
* 2 ml FCS | |||
* 500 ml filter with bottle | |||
== Procedure == | == Procedure == | ||
# put all together and filtrate all | |||
# label all with Low Serum, name and date | |||
Latest revision as of 14:26, 8 June 2015
Material
- 280 ml MEM
- 100 ml sterile water
- 1 tube Pen/ Strep (4ml)
- 1 tube Glutamine (4ml)
- 1 tube Insulin (4 ml)
- 2 ml FCS
- 500 ml filter with bottle
Procedure
- put all together and filtrate all
- label all with Low Serum, name and date