Preparation of High Serum for C2C12 cells: Difference between revisions

Latest revision as of 14:34, 8 June 2015

thaw all frozen stuffs at 37 ºC
add in the Stericup the 40 ml FCS, 4 ml pen/ strep and the 4 ml glutamine (if necessary)
fill this up to 400 ml with the DMEM
filtrate the Medium in the bottle by connecting the vacuum pump to the steritop
label the bottle with the name, the date and “high serum for C2C12”

@@ Line 1: / Line 1: @@
-Materials:
+== Material ==
--	1 tube pen/strep (green, 4 ml)
+* 1 tube pen/strep (green, 4 ml)
--	1 tube glutamine (blue, 4 ml)
+* 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax
-→ this is not necessary if the DMEM has glutamax
+* 1 bottle DMEM with 4500 mg/ l glucose
--	1 bottle DMEM with 4500 mg/ l glucose
+* 40 ml FCS (frozen in a 50 ml FALCON tube)
--	40 ml FCS (frozen in a 50 ml FALCON tube)
+* 1 large Stericup (500 ml) (bottle + filter + top)
--	1 large Stericup (500 ml) (bottle + filter + top)
-procedure:
+== Procedure ==
--	thaw all frozen stuffs at 37 ºC
+# thaw all frozen stuffs at 37 ºC
--	add in the Stericup the 40 ml FCS, 4 ml pen/ strep and the 4 ml glutamine (if necessary)
+# add in the Stericup the 40 ml FCS, 4 ml pen/ strep and the 4 ml glutamine (if necessary)
--	fill this up to 400 ml with the DMEM
+# fill this up to 400 ml with the DMEM
--	filtrate the Medium in the bottle by connecting the vacuum pump to the steritop
+# filtrate the Medium in the bottle by connecting the vacuum pump to the steritop
--	label the bottle with the name, the date and “high serum for C2C12”
+# label the bottle with the name, the date and “high serum for C2C12”