Serum immunostaining: Difference between revisions

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(Created page with "== Responsible person == If you have any troubles please contact Dunja. == Protocol == The following protocol applies to '''Gibco Goat Serum''' from Invitrogen (Ordernr.: 16...")
 
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== Protocol ==
== Protocol ==
The following protocol applies to '''Gibco Goat Serum''' from Invitrogen (Ordernr.: 16210).
The following protocol applies to '''Gibco Goat Serum''' from Invitrogen (Order No.: 16210).


<ol>
<ol>
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<li>Cool on ice and aliquot into 50ml Falcon tubes.</li>
<li>Cool on ice and aliquot into 50ml Falcon tubes.</li>
<li>Keep at -20 C</li>
<li>Keep at -20 C</li>
</ul>
</ol>


For detailed information see Heat Inactivation of Serum.
For more information see the [[Media:Heat_Inactivation_of_Serum.pdf|Detailed protocol]].

Latest revision as of 15:54, 8 November 2012

Responsible person

If you have any troubles please contact Dunja.

Protocol

The following protocol applies to Gibco Goat Serum from Invitrogen (Order No.: 16210).

  1. Defrost the serum (takes a long time- maybe overnight in the fridge is practical)
  2. Heat inactivate for 30 min at 56C
  3. Cool on ice and aliquot into 50ml Falcon tubes.
  4. Keep at -20 C

For more information see the Detailed protocol.