Preparation of Freezing media for C2C12 cells: Difference between revisions

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== Materials ==
== Material ==


* 25 ml FCS (50%)
* 25 ml FCS (50%)
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== Execution ==
== Procedure ==


# put all stuffs together  
# put all stuffs together  
# filtate the medium and aliquote the freezing media into 4 ml per tube
# filtate the medium and aliquote the freezing media into 4 ml per tube
# store at –20ºC
# store at –20ºC

Latest revision as of 14:34, 8 June 2015

Material

  • 25 ml FCS (50%)
  • 20 ml DMEM + Pen/ Strep + Glut (40%)
  • 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro


Procedure

  1. put all stuffs together
  2. filtate the medium and aliquote the freezing media into 4 ml per tube
  3. store at –20ºC