Preparation of High Serum for C2C12 cells: Difference between revisions
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(Created page with "Materials: - 1 tube pen/strep (green, 4 ml) - 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax - 1 bottle DMEM with 4500 mg/ l glucose - 40 ml...") |
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Materials | == Materials == | ||
* 1 tube pen/strep (green, 4 ml) | |||
* 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax | |||
→ this is not necessary if the DMEM has glutamax | * 1 bottle DMEM with 4500 mg/ l glucose | ||
* 40 ml FCS (frozen in a 50 ml FALCON tube) | |||
* 1 large Stericup (500 ml) (bottle + filter + top) | |||
== Execution == | |||
# thaw all frozen stuffs at 37 ºC | |||
# add in the Stericup the 40 ml FCS, 4 ml pen/ strep and the 4 ml glutamine (if necessary) | |||
# fill this up to 400 ml with the DMEM | |||
# filtrate the Medium in the bottle by connecting the vacuum pump to the steritop | |||
# label the bottle with the name, the date and “high serum for C2C12” | |||
Revision as of 08:58, 25 March 2015
Materials
- 1 tube pen/strep (green, 4 ml)
- 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax
- 1 bottle DMEM with 4500 mg/ l glucose
- 40 ml FCS (frozen in a 50 ml FALCON tube)
- 1 large Stericup (500 ml) (bottle + filter + top)
Execution
- thaw all frozen stuffs at 37 ºC
- add in the Stericup the 40 ml FCS, 4 ml pen/ strep and the 4 ml glutamine (if necessary)
- fill this up to 400 ml with the DMEM
- filtrate the Medium in the bottle by connecting the vacuum pump to the steritop
- label the bottle with the name, the date and “high serum for C2C12”