Thawing (XB10) hybridoma cells: Difference between revisions
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1 x 15 ml conical tube per vial of frozen cells
water bath (37 C for mammalian cells)
High serum media (RPMI media +10% FCS+glutamine+P/S) [****Use Defined Supplemented Calf Serum (DSCS) from Hyclone (Prebio) for making high serum media for hybridoma cells]
1 blue cap flask
1 x 10 ml pipettes
gestopfft pasteur pipettes
ungestopfft pasteur pipettes
frozen cells in liquid nitrogen or dry ice.
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==Materials:== | ==Materials:== | ||
<li>1 x 15 ml conical tube per vial of frozen cells | |||
<li>water bath (37 C for mammalian cells) | |||
<li>High serum media (RPMI media +10% FCS+glutamine+P/S) [****Use Defined Supplemented Calf Serum (DSCS) from Hyclone (Prebio) for making high serum media for hybridoma cells] | |||
<li>1 blue cap flask | |||
<li>1 x 10 ml pipettes | |||
<li>gestopfft pasteur pipettes | |||
<li>ungestopfft pasteur pipettes | |||
<li>frozen cells in liquid nitrogen or dry ice. | |||