RNA Extraction from axolotl tissue: Difference between revisions
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Trizol (Invitrogen)
Chloroform
Isopropanol
RNase free water
Potter homogenizer plus appropriate accessories (Stoessel und Pistille)
5 ml syringe
25 guage needle
Autoclaved Eppendorf tubes
75% ethanol in DEPC water
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<li>Autoclaved Eppendorf tubes</li> | <li>Autoclaved Eppendorf tubes</li> | ||
<li>75% ethanol in DEPC water</li> | <li>75% ethanol in DEPC water</li> | ||
<h3>Procedure to extract total RNA:</h3> | |||