View source for Preparation of Freezing media for A1 cells

Materials
* DMSO (one ampules from Sigma)
* High serum for A1 cells
* 2x 50 ml FALCON tubes
* 1x 15 ml FALCON tube
* Pipettes
** 10x 5 ml FALCON tubes

Procedure
-	clean the ampuls with ethanol
-	transfer the DMSO into the 15 ml FALCON tube
-	pipette in each 50 ml FALCON tube 18 ml High serum
-	put into each 2 ml DMSO and mix 
-	aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube)
-	store the tubes into –20ºC