View source for Thawing frozen C2C12 cells

== Materials ==
* frozen C2C12 cells
* HS for C2C12 cells
* pipettes and tips
* FALCON tube 15 ml
* Cell culture flask

== Procedure ==
# heat the HS up to 37ºC
# pipette 14 ml C2C12 HS media in a 75 cm2 cell culture flask
# pipette carefully 1 ml from the warm media in the cryo tube to the frozen cells
# pipette carefully up and down till the cells are thaw
# pipette all in the FALCON tube
# centrifuge at 1000 xg, 3 min at 4ºC
# label the cell culture flask with the name, date and C2C12 with the passage number
# aspirate of the media and resuspend the cells in 1 ml of the new HS 
# pipette the suspension in the cell culture flask
# check the cells under the microscope
# put the flask at 37ºC, 10% CO2