View source for Thawing 293FT cells

→ thaw the cells in prewarmed , complete medium without Geneticin

== Materials ==
* a vial of frozen cells from the liquid nitrogen
* 37ºC waterbath
* 75 cm2 flask
* HS for 293 FT cells
* pipetts

== Procedure ==
# remove the vial of frozen cells from liquid nitrogen and thaw quickly in a 37ºC waterbath
# transfer the cells to a 75 cm2 flask containing 12 ml Hs for 293 FT cells w/o Geneticin
# incubate the flask for 2 –4 hours to allow the cells to attach to the bottom of the flask
# aspirate off the medium and replace with 12 ml of fresh, complete medium without  Geneticin
# incubate the cells over night
# the next day, aspirate off the medium and replace with fresh, complete medium containing 500 µg/ ml Geneticin
# incubate the cells and check them daily until the cells are 80 – 90% confluent
# proceed to subculturing cells