View source for Freezing of C2C12 cells

==Materials:==
* 15 ml FALCON tube
* PBS w/o Ca2+ and Mg2+
* TE in PBS
* Freezing media (10% DMSO + 40% serum + 50% DMEM)
* Pipettes and tips
* Glas pipetts
* cryo tube
* ice
* freezing box

==Procedure:==
-	wash the cells with 5 ml PBS
-	remove the PBS and add 2 ml TE
-	let it incubate for 3 – 5 min
-	stop the TE with 5 ml HS – media and resuspend the cells 
-	pipette the cell suspension back in the FALCON tube
-	centrifuge the cells (1000 xg, 3 min at 4 ºC)
-	remove the old media 
-	resuspend the cells 1 ml freezing media
-	quickly pipette the liquid in the cryo tube
-	put the cells in the freezing box and store this for one day at –80 ºC
-	place the cells one day later into the liquid nitrogen